Aim: Urinary tract infections are in the first ranks in community acquired or nosocomial infections. In this work we aimed to investigate the most frequent isolated bacteria and their antibiotic susceptibilities in urine samples. Material and Method: Urine samples sent to Microbiology Laboratories of Tavsanli State Hospital and Usak State Hospital between January 2011 to June 2011 were retrospectively analysed. Bacterial isolation from clinical samples was made using standard microbiological methods. Antimicrobial susceptibility was assessed using disk-diffusion methods according to CLSI recommendations. Result: E. coli was the most frequent isolated bacteria in both hospitals. The other common bacteria in Tavsanli were Pseudomonas aeruginosa and Klebsiella spp., and in Usak Enterococcus spp. and Klebsiella spp. respectively. The most effective antibiotics against Gram negative enteric bacteria were imipenem and amikacine, against Pseudomonas spp. were ceftasidime and amikacine. The most effective antibiotic against Gram positive bacteria is vancomycin. Discussion: The identification of the microorganisms and the nature of their antimicrobial susceptibilities isolated from urinary tract infection is thought to be important for medical centers to determine their own antimicrobial policies.