Akademik Veri Yönetim Sistemi
BALKAN ACADEMY OF FORENSIC SCIENCES 14th ANNUAL SCIENTIFIC MEETING, İstanbul, Türkiye, 5 - 08 Ekim 2023, ss.121
Analytical
Approaches to Examining PSA (Prostate Specific Antigen) Concentration in Semen
Samples
Mustafa İREGÜR¹, Belgin İZGݲ
¹Bursa Uludağ
University, Graduate School of Natural and Applied Sciences, Department of
Forensic Sciences, Bursa, Turkey
²Bursa Uludağ University, Department of
Chemistry, Department of Analytical Chemistry, Bursa, Turkey
502240007@ogr.uludag.edu.tr
Background and Aim:
Forensic sciences play a major role in sexual assault cases. It is of great
importance to be able to detect the semen of the accused in the body cavities
of the victim, on his clothes or at the crime scene, in order to definitively
prove the sexual assault allegation and to determine the identity of the
aggressor. The semen in the body cavities will either remain in traces or
disappear as it is diluted by drainage or by washing the person. Two methods
are used to detect this semen found. The first of these is the method based on
direct microscopic examination. The second is the method of showing the
presence of semen by searching for a chemical compound or enzyme depending on
the chemical reaction.
Method and Material:
PSA concentration measurement, which is an important marker in the detection of
semen and included in the second method, has been successful in proving the
presence of semen. In studies related to this, either rapid antigen test kits
or ELISA method were used. In this study, PSA concentration measurements will
be made with Cobas E402 analyzer in various dilutions of semen samples taken
from volunteers.
Conclusion : In our study, unlike
other methods, the ECLIA method will be used, and the results to be obtained
from this method and the usability of this method will be discussed, and the
advantages and disadvantages of the application will be determined.
Discussion : In order to
eliminate the high-dose hook effect that will occur if the semen is studied
without diluting, the sample should be diluted and studied. Since PSA is found
in some other body fluids, albeit at very low levels, the important thing is
not that the sensitivity limit of the test is very low, but that it is at a
point where it can distinguish the antigen in other body fluids from the
antigen in semen.
Bursa Uludağ University
Faculty of Medicine Clinical Research Ethics Committee has approved the ethics
committee with the decision number 2023-3/13 dated 14 February 2023.
Keywords: Sexual assault,
semen, ECLIA, PSA