Fractionation Analysis of Manganese in Turkish Hazelnuts (Corylus avellana L.) by Inductively Coupled Plasma-Mass Spectrometry


SEVEN ERDEMİR Ü. , Gucer S.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, vol.62, no.44, pp.10792-10799, 2014 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 62 Issue: 44
  • Publication Date: 2014
  • Doi Number: 10.1021/jf503145t
  • Title of Journal : JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
  • Page Numbers: pp.10792-10799
  • Keywords: hazelnut (Corylus avellana L.), manganese, fractionation, bioaccessibility, ICP-MS, SIZE-EXCLUSION CHROMATOGRAPHY, TRACE-ELEMENTS, FATTY-ACIDS, VARIETIES, BIOAVAILABILITY, EXTRACTION, MINERALS, LIPIDS, SERUM, BORON

Abstract

In this study, an analytical fractionation scheme based on water, diethyl ether, n-hexane, and methanol extractions has been developed to identify manganese-bound fractions. Additionally, in vitro simulated gastric and intestinal digestion, n-octanol extraction, and activated carbon adsorption were used to interpret the manganese-bound structures in hazelnuts in terms of bioaccessibility. The total content of manganese in the samples was determined by inductively coupled plasma-mass spectrometry after microwave-assisted digestion, and additional validation was performed using atomic absorption spectroscopy. Water fractions were further evaluated by high-performance liquid chromatography hyphenated to inductively coupled plasma-mass spectrometry for the identification of water-soluble manganese fractions in hazelnut samples. The limits of detection and quantification were 3.6 and 12.0 mu g L(-1), respectively, based on peak height.