Investigation of the Release of Growth Factors from Nanofiber Mat Loaded with Apheresis Platelet Concentrate (APC)


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Yılmaz H., Aras C., Karaçay M., Altuntuğ Cesur M. İ., Karaca E., Temel Ş. G., ...More

International Congress on Biological and Health Sciences, Afyonkarahisar, Turkey, 26 February 2022

  • Publication Type: Conference Paper / Summary Text
  • City: Afyonkarahisar
  • Country: Turkey
  • Bursa Uludag University Affiliated: Yes

Abstract

Investigation of the Release of Growth Factors from Nanofiber Mat Loaded with Apheresis Platelet Concentrate (APC) #

 

Hülya Yılmaz1,2, Cansu Aras3, Mehmet Karaçay4, Merve İlkay Altuntuğ Cesur5, Esra Karaca3, Şehime Gülsün Temel6,7,8, Emel Bülbül Başkan9, Barbaros Oral4, Ekrem Kaya1

 

1Department of General Surgery, Faculty of Medicine, Bursa Uludag University, Gorukle, 16059 Bursa, Turkey

2Department of Translational Medicine, School of Health Sciences, Bursa Uludag University, Bursa, Turkey

3Department Textile of Engineering, Faculty of Engineering, Bursa Uludag University, Gorukle, 16059 Bursa, Turkey

4Department of Immunology, Faculty of Medicine, Bursa Uludag University, Gorukle, 16059 Bursa, Turkey

5Department of Biomaterials, Graduate School of Natural and Applied Science, Bursa Uludag University, 16059 Bursa, Turkey

6Department of Medical Genetics, Faculty of Medicine, Bursa Uludag University, 16059 Bursa, Turkey

7Department of Histology & Embryology, Faculty of Medicine, Bursa Uludag University, 16059 Bursa, Turkey

8Department of Translational Medicine, Institute of Health Sciences, Bursa Uludag University, Bursa, Turkey

9Department of Dermatology, Faculty of Medicine, Bursa Uludag University, Bursa, Turkey

 

 

 

Corresponding author: hlyylmz2017@gmail.com

 

 

 

Abstract:

 

Introduction: In this study, the production of APC-loaded nanofiber mats using the electrospinning method and examining the release of growth factors from the produced APC-loaded nanofiber mats was planned. Materials and Methods: APC-loaded composite nanofiber mats (n=5) were produced using the electrospinning system in textile engineering laboratory. The surfaces morphologies of the produced mat were examined using a scanning electron microscope. Studies for release were carried out at department of immunology. PBS (0.5 mL) was collected at 0, 48, 96, 144, 192, 240, 288 and 336 hour intervals for the analysis of growth factors to be released into PBS from APC-loaded nanofiber mat. Collected PBSs for quantitative analysis of growth factors and BCA (Bichchoninic acid) protein concentration analysis were stored at -20oC for analysis. The growth factors released from APC-loaded composite nanofiber mat into PBS at certain time intervals,

1- Epidermal growth factor, EGF;

2- Basic fibroblast growth factor, bFGF;

3- Platelet-Derived Growth Factor AA (PDGF-AA)

quantitative analyzes were performed with the technology laboratory ELISA Kit (Elabscience, USA) as recommended by the manufacturer. Results: When the release amounts between FGF, EGF and PDGF groups were compared, a significant difference was found in all time periods from the 48th hour to the 336th hour (respectively, p=0.004, p=0.008, p=0.006, p=0.009, p=0.009, p=0.009; p<0.05). When the time-dependent release changes within each group were examined, there was no statistically significant difference in the FGF group (p=0.084; p>0.05), but there was a significant difference between the EGF and PDGF groups (p=0.012, p=0.016; p<0.05). It was observed that there was a significant difference between the 48th hour and the 288th and 336th hours in the EGF group, and lower release occurred at these hours. In the comparison made for PDGF, it was seen that there was a significant difference between the 48th hour and the 192nd and 288th hour, and lower release was realized at these hours. Conclusion: It is thought that the results to be obtained from the research are useful in terms of using APC-loaded nanofiber mats as a wound dressing in future research and providing scientific data. 

 

Keywords: growth factors, nanofiber, release, apheresis platelet concentrate.

 

 

 

# The study financially supported as research project No THIZ-2021-471 by the decision of Bursa Uludag University Scientific Research Project Coordinatorship. The budget approved for our project was 9.576,90 Turkish liras.