Evaluation of serum anti-Mullerian hormone concentrations following treatment with vitamin D in dairy heifers.

Koca D., Nak Y., Şendağ S., Nak D., Arslan T., Wehrend A.

56th Annual Conference of Physiology and Pathology ofReproduction and 48th Joint Conference of Veterinaryand Human Reproductive Medicine, Münster, Germany, 1 - 03 March 2023, vol.58, no.2, pp.21

  • Publication Type: Conference Paper / Summary Text
  • Volume: 58
  • City: Münster
  • Country: Germany
  • Page Numbers: pp.21
  • Bursa Uludag University Affiliated: Yes


Anti­Müllerian hormone (AMH) levels in theblood are positively associated with numberof antral follicles, ovarian function, and fertil­ity. Numerous studies, particularly in humanmedicine, have shown that vitamin D (VD)has a positive effect on AMH levels, both di­rectly and indirectly. To our knowledge, theeffects of VD on serum AMH levels inheifers have not been investigated. Therefore,the cur­ rent study aimed to investigate thefollowing question: Is VD treatmentassociated with in­ creased serum AMHconcentrations in dairy heifers? The studyincluded twenty healthy non­pregnant dairyHolstein heifers (11–13 months old)weighing an average of 319 kg (288–341kg). The animals did not have direct contactwith sunlight during the study be­ causethere were no open areas in the facility. Inother standard rations (NRC) no vitamins orother additional nutrients were supplied to theanimals. All heifers received a single intra­muscular dosage of 5 ml (5 million IU) VD(Provet­D3®, Provet, Istanbul­Turkey).Blood samples were collected from the tailveins of all animals before (day 0) and after(days 7, 14, and 28) VD injection. Theelectrochemilu­ minescence immunoassaymethodology was used to measure AMH.Comparison results showed that the AMHconcentrations in the t0, t7, t14, and t28 dayswere not statistically different from eachother’s (p = 0.10). Also, it was seen thatthere were no statistically sig­ nificantcorrelations between the pairs (age­ AMH)and (weight­AMH) in the t0 day, and (VDlevel­AMH) for all days. Grants: Thesefindings demonstrated that exogenous VD didnot affect on AMH in heifers.