Comparison of IgG and semiquantitative tests for evaluation of passive transfer immunity in calves


TOPAL O. , BATMAZ H. , MECİTOĞLU Z. , UZABACI E.

TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES, vol.42, no.4, pp.302-309, 2018 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 42 Issue: 4
  • Publication Date: 2018
  • Doi Number: 10.3906/vet-1712-43
  • Title of Journal : TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES
  • Page Numbers: pp.302-309
  • Keywords: Calf, immunity, Brix refractometer, total protein, gamma-glutamyl transferase, glutaraldehyde coagulation test, GLUTARALDEHYDE COAGULATION TEST, IMMUNOGLOBULIN-G CONCENTRATION, DAIRY CALVES, BRIX REFRACTOMETER, COLOSTRUM QUALITY, PROTEIN-CONCENTRATION, BOVINE COLOSTRUM, BEEF-CALVES, SERUM, FAILURE

Abstract

Serum immunoglobulin (IgG) and semiquantitative tests are used for the evaluation of passive transfer immunity (PTI) in calves. We aimed to evaluate PTI in calves by using a Brix refractometer, total protein (TP), gamma-glutamyl transferase (GGT), and glutaraldehyde coagulation test (GCT) on days 1, 3, and 7 after birth; to compare the results with serum IgG; and to evaluate which day these tests are given on will give the best results. The blood samples were collected from 60 Holstein dairy calves on day 0, just after birth, and on the 1st, 3rd, and 7th days after birth. The serum IgG concentration was measured by ELISA, the Brix % and TP concentrations with refractometers, and GGT activity using a dry chemistry system. The duration of the GCT was determined in the first 60 min. The IgG, TP concentration, and Brix % all peaked on the 3rd day of the study. GGT showed a significant decline after 24 h. Evaluating the Brix %, IgG, TP, and GCT levels more than 24 h after birth gives better results. However, GGT activity was observed as an early indicator of failure of passive transfer, as the GGT levels were highest 24 h after birth.