We investigated the effects of Ankaferd Blood Stopper (ABS) and tripeptide copper complex (TCC) on wound healing in rats. A total of 24 outbred, male, Sprague-Dawley rats were randomly divided into (1) ABS, (2) TCC, and (3) control groups. Bilateral experimental wounds were created near the caudal border of the scapula. Each wound in the respective treatment group was treated daily with sponges soaked in ABS solution, topical TCC gel, or saline. On days 0, 7, 14 and 21, unhealed wound area was measured and biopsy samples were taken for histopathological analysis (except day 0). Median time for the first observable granulation tissue was not significantly different in the ABS, TCC, and control groups (5.8, 5.5, and 6.7 days, respectively) (P>0.05). Filling of the open wound with granulation tissue to skin level was significantly slower in the control group than in the ABS and TCC groups (18, 9, and 11 days, respectively). The mean unhealed wound area was significantly smaller and the mean percentage of total wound healing was significantly higher in the ABS- and TCC-treated wounds than in the control wounds on day 7, 14, and 21 (P<0.05); the average time for healing was also significantly shorter in the treatment groups than in the control group (17.4, 16.8 vs. 23.6 days, respectively) (P<0.05). Our results suggest that topical application of ABS and TCC have beneficial effects on wound healing.