LncRNA-based approach to predicting of recurrence after liver transplantation in hepatocellular carcinomas


Aksoy S. , Aksoy F. , Dündar H. Z. , Tunca B. , Uğraş N. , Kaya E. , ...More

55th Congress of the European Society for Surgical Research, Innsbruck, Austria, 10 - 11 December 2020, pp.152-153

  • Publication Type: Conference Paper / Summary Text
  • City: Innsbruck
  • Country: Austria
  • Page Numbers: pp.152-153

Abstract

Aim: Despite liver transplantation (LT) remaining one of the most effective treatments, a signicant proportion of patients still suffer from unfavorable outcomes due to a high recurrence rate. Studies have shown that noncoding RNAs plays a vital role in poor prognosis. Thus, the aim of the present study was to evaluate the relation of Long noncoding RNAs (LncRNAs) expression levels and the histopathological features of tumors in recurrence formation in HCC cases. 

Methods: Between September 2008 and January 2015, 102 HCC patients who underwent LT in Uludag University Hospital were enrolled. The relationship between the altered expression of 15 individual metastasis-related LncRNAs, clinical and pathological features were assessed. 

Results: Compared with non-recurrent patients, among 15 LncRNAs, the expression level of HOTAIR, MALAT1 and NEAT1 were 3.2-fold, 4.-fold, 2.8-fold higher, H19 was 2.2-fold lower in patients with recurrence during the follow-up (p = 0.0301, P=0.0234, p= 0.0231 and p = 0.0101, respectively). In addition, according to multivariate cox regression models, the coexistence of high levels of MALAT1 expression, high expression of HOTAIR and diagnosis of NAFLD and tumor size were associated with recurrence (p < 0.001). Recent studies have shown that LncRNAs play a crucial role in tumorigenesis. These RNA molecules have been found to regulate important processes, such as development, differentiation, metastasis, and drug resistance in cancer tumors. Currently, the dysregulation of several LncRNAs have been identified in HCC. The current study has several limitations. We investigated only 15 LncRNAs that play specific biological roles by using a qRT-PCR. Although the current study included a homogeneous and carefully selected group of patients, its limitation was the relatively small group of patients who were analyzed. We evaluated LncRNA expression using a clinical specimen; however, in vitro and animal studies are still required. 

Conclusion: We suggest that the combination of induced MALAT1 and HOTAIR expressions promising biomarkers for the prediction of recurrence in HCC patients after LT. Using these markers, a new scoring system can be designed to detect recurrence in patients without poor prognostic factors.