© 2017 IJVS.This study aimed to evaluate some hematologic, sero-biochemical parameters and microbiological culture results of umbilical lesions in calves. Holstein breed, six calves (n=6) at different age were presented with the complaint of umbilical lesions at different times. Clinical parameters of the cases were recorded, and umbilical lesions were diagnosed following to clinical and ultrasonographical examinations. The encountered pathologies were omphaloarteritis and omphalourachitis in three cases, omphalophlebitis in a case, ulcerous omphalitis in a case and omphalophlebitis and polyarthritis in a case. Peripheral blood samples were collected and then some hematological and sero-biochemical parameters were analyzed. The microbiological samples were taken from umbilical lesions for culture. Blood sample analyses revealed the systemic inflammatory response syndrome and microbiological culture results pointed out enteric bacteria (Escherichia coli, Proteus spp., Pseudomonas putida, Pseudomonas stutzeri, Providencia stuartii, Proteus mirabilis). As a conclusion, it may be emphasized that a brief hematological, serobiochemical evaluation as well as local microbiological culture results of the umbilical lesions have valuable information both determination of the prognosis and evaluation of the healthy status of the calves.