The effect of volatile oil mixtures on the performance and ilio-caecal microflora of broiler chickens

Cetin E., Yibar A., YEŞİLBAĞ D., Cetin I., CENGİZ Ş. Ş.

BRITISH POULTRY SCIENCE, vol.57, no.6, pp.780-787, 2016 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 57 Issue: 6
  • Publication Date: 2016
  • Doi Number: 10.1080/00071668.2016.1214682
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.780-787
  • Bursa Uludag University Affiliated: Yes


1. The aim of this study was to investigate the individual and combined effects of rosemary, oregano and fennel volatile oil (FVO) supplementation on the performance and ilio-caecal bacteriological flora of broiler chickens.A total of 800 male Ross-308 broiler chickens were divided equally into 8 groups; each contained 100 chickens. The study included a control treatment (NC) with no dietary additives that was supplemented with oils according to the following 7 treatments: 200mg -tocopherol acetate/kg (PC), 100mg oregano volatile oil (OVO)/kg, 100mg rosemary volatile oil (RVO)/kg, 100mg FVO/kg and an equal mixture of oregano+rosemary+fennel VO (100, 200, 400mg/kg, VOM-1, VOM-2 and VOM-3, respectively). The experiment lasted for 6weeks.At the end of the experiment, dietary supplementation with -tocopherol, oregano, rosemary and FVO and two different volatile oil mixtures (VOMs) (VOM-2; VOM-3) significantly increased the body weights (BWs) of broilers at 7, 14 and 21d of age compared to the negative control (NC) (-) and VOM-1 groups. At 0-42d, birds fed on VOM-3 were considerably heavier and also gained more weight than NC (-) and VOM-1 groups.The blend of VOs at 400mg/kg significantly increased Lactobacillus spp. in faeces. The blends of oregano, rosemary and FVOs (VOM-3) at 400mg/kg concentration and also VOM-3 group exhibited stronger antibacterial activity against coliform bacteria compared to the NC (-) group.In conclusion, the blend of oregano, rosemary and fennel VOs at higher concentrations (400mg/kg concentration) in diets can be used to stimulate the growth and can improve the intestinal microbial balance (including a reduction of coliform bacteria and an increase in Lactobacillus spp. counts) of broiler chickens.