Tarantula cubensis extract (Theranekron® ) ınhibits ınflammation in carrageenan-ınduced acute paw edema in rats

Özfırat E. C. , Topal A. , Akkoç A. , Gül Satar N. Y. , İpek V., Pamukçular Ö., ...More

Arquivo Brasileiro De Medicina Veterinaria E Zootecnia, vol.74, no.1, pp.56-64, 2022 (Journal Indexed in SCI Expanded)

  • Publication Type: Article / Article
  • Volume: 74 Issue: 1
  • Publication Date: 2022
  • Title of Journal : Arquivo Brasileiro De Medicina Veterinaria E Zootecnia
  • Page Numbers: pp.56-64


The aim of this study was to investigate the anti-inflammatory effect of alcoholic extract of Tarantula cubensis

alcoholic extract (TCAE) in experimentally induced inflammation in rats. Fifty-four adult Sprague-Dawley

male rats were randomly divided into nine groups. Paw edema was induced by 0.2mL subplantar (s.p.)

injection of 1% carrageenan (CAR) into the right hind paw. Rats were treated with the nonsteroidal anti-

inflammatory drug (NSAID) indomethacin (INDO) (10mg/kg, p.o.) or TCAE at different doses (1, 10 or

100μg/kg) injected s.c. for systemic or s.p. for local anti-inflammatory effect. Saline was used as control.

Changes in paw thickness, volume, and weight were calculated as percentages. Formalin-fixed paws were used

for histopathological examination. We detected that TCAE applied s.c. at 10μg/kg and 100μg/kg doses resulted

in thinner paw thickness, lower paw volume, and lower paw weights four hours after the induction of

inflammation when compared with the INDO group (p<0.05). The paw edema inhibitory effect of TCAE

applied at a dose of 10μg/kg, s.c. was 68% when compared with the INDO which had an inhibitory effect of

56%. These results were verified with similar histopathological findings. The anti-inflammatory feature of

10μg/kg of TCAE given systematically was similar to the effects of INDO. Our results suggest that TCAE has

anti-inflammatory effects by reducing edema and decreasing inflammatory reaction. These results may be

attributed to the inhibition of the production of proinflammatory mediators. Thus, TCAE may be considered as

a potential anti-inflammatory agent for treating acute inflammatory conditions.