Akademik Veri Yönetim Sistemi
Alver O., Heper Y.(Yürütücü), Ercan İ., Akalın E. H., Töre O., Töre (Emekli) O.
Yükseköğretim Kurumları Destekli Proje, 2003 - 2005
This study was designed to determine the prevalence of intestinal parasites in relation to personal and
environmental risk factors in Bursa province of Turkey and to compare wet mount + Lugol's iodine,
formalin ethyl acetate concentration, trichrome staining and enzyme-linked immunosorbent assays
(ELISA) methods used in Entamoeba histolytica/ Entamoeba dispar diagnosis. For this purpose a total
of 176 faecal samples from people were randomly collected from regions of the Bursa City Centre,
where crowded families of low socio-economic levels reside. All faecal samples were examined by wet
mount + Lugol's iodine formalin ethyl acetate concentration, trichrome staining methods and ELISA. 66
faecal samples were infected with one or more parasite species and the overall prevalence rate was
37.5%. Nine species of intestinal parasites, including six protozoon and three helminth species were
found. The most prevalent species was Blastocystis hominis (17.04%) and others were found with the
following frequencies: 9.09% Enterobius vermicularis, 7.38% Giardia intestinalis, 5.68% Entamoeba coli,
2.27% E. histolytica/E.dispar, 1.13 % Entamoeba hartmani, 1.13% Taenia spp., 0.56% Hymenolepis nana.
6.2% of examined samples had mixed infections. Overall prevalence of the detected parasites did not
differ significantly in different demographic groups. Except for B. hominis and G. intestinalis, there was
no evidence for sex, age and other demographic-related differences in the prevalence of these
parasites. Statistically, B. hominis and G. intestinalis were higher in 20 to 49 and in 1 to 19 year age
groups, respectively than in any other age group. Microscopical examination and ELISA revealed that
one sample (0.5%) was found to be positive using all 4 methods, and 162 samples (92%) were found to
be negative with all 4 methods applied. E. histolytica/E. dispar complex was detected in 0.56% (1); in
0.56% (1), in 0.56% (1) and in 2.27% (4) of the fecal samples examined by wet mount + Lugol's iodine,
formalin ethyl acetate concentration, with trichrome staining, and ELISA respectively. The wet mount +
Lugol's iodine, formalin ethyl acetate and trichrome staining methods had a sensitivity of 25% each, a
specificity of 94.1, 99.4 and 98.2%, compared to the results of the E. histolytica/E. dispar ELISA,
respectively.