İnvaziv duktal karsinom teşhisi almış meme kanseri hastalarında yap/TAZ/ MST/LATS gen ekspresyonlarının retrospektif olarak araştırılması

Thesis Type: Postgraduate

Institution Of The Thesis: Uludağ Üniversitesi, Turkey

Approval Date: 2019

Thesis Language: English




Breast cancer is the most frequent cancer type in women. When we consider the increase in its the frequency and the decrease in age of diagnosed patients, we realize that the importance of a screening program and breast cancer awareness is increasing day by day in our country. Also, treatments for breast cancer have many limitations, such as resistance and a lack of reliable biomarkers. Recent studies have also shown that aberrant Hippo pathway functioning can drive tumor formation and breast cancer metastasis due to its critical role in regulating cellular proliferation and organ size. This signaling controls cancer development, cell proliferation, and regulation of apoptosis. However, the detailed molecular mechanism that regulates the Hippo signaling in breast cancer metastasis, and the association of Hippo pathway components levels with clinicopathological characteristics are yet to be fully elucidated. In the present study, the Hippo pathway member levels (YAP1, TAZ, LATS1/2, MST1/2) in 100 patients with IDC breast cancer analyzed, and the association of Hippo pathway components levels with survival and clinicopathological characteristics of patients by RT-PCR were assessed. Our study showed that YAP1, LATS1, and LATS2 were significantly (p= 0,000; p= 0,0004; p= 0,0001; respectively) down-regulated in tumor tissues. Also, in this study observed significant association between the expression of low-YAP with PR status, HER2 status, tumor grade, Ki-67 proliferation index, metastasis tumors, lymph node, and tumor size. As well as, in the present study demonstrated significant association between the expression of low-LATS2 with Ki-67 proliferation index, metastasis tumors, and lymph node. In conclusion, studies are shown that the Hippo pathway plays an important role in the development of breast cancer by combining the clinical data obtained from the experimental studies in the literature.