Determination of leaf rust (puccinia triticina) resistance genes in bread wheat (triticum aestivum l.) using simple sequence repeats (ssrs) markers


Thesis Type: Doctorate

Institution Of The Thesis: Bursa Uludağ University, Turkey

Approval Date: 2019

Thesis Language: Turkish

Student: Pakize Özlem Kurt Polat

Consultant: KÖKSAL YAĞDI

Abstract:

In this study wheat varieties that are largely used in Turkey, CIMMYT Thatcher genotypes that are supplied from Mexico and the F1 lines that have been obtained by hybridization of these, were used as experiment materials. SSR (Simple Sequence Repeats- Microsatellite) method was used to determine the existence of Lr10 and Lr19 genes which are the genes for resistance to leaf rust disease of wheat. The study was carried out in the experimental field and in the Seed Laboratory of the Bursa Uludag University, Agricultural Faculty of the Field Crops Department. Genotypes used in the study have been cultivated in trial areas in 2013-2017 growing seasons in order to increase experiment materials, to perform the hybridization between genotypes and to perform land observations of leaf rust disease. 13 F1 lines that have been obtained by hybridization, 22 wheat variety for bread-making (Triticum aestivum L.) and 3 Thatcher lines (Lr9, Lr10, TcHassas), have been put through DNA analysis by using the SSR (Microsatellite) method. According to the results Lr10 gene was identified in Karatopak, Kaşifbey ve Gün-91 varieties and the Lr19 was detected in Karatopak, İzmir-85, Ceyhan-99, Aldane, Flamura-85 varieties and in the Aldane x Karatopak, Flamura x Lr19 F1 lines. It has been established that SSR methods are usable for the research of genes for resistance to leaf rust in wheat. Also, since the Thatcher (isogenic lines) lines used in the study have a narrow genetic structure it has been concluded that they are good marker genomes for determining the scanned resistance genes .It has also been concluded that in future breeding programs that will be carried out, for determining the genotypes that have resistance genes, using molecular makers will be efficient in reaching faster and more precise results.