Molecular analysis of the effects of glucose and nitrogen signal transduction pathways on the transcription of nth1 gene


Thesis Type: Postgraduate

Institution Of The Thesis: Bursa Uludağ University, Fen Bilimleri Enstitüsü, Biyoloji , Turkey

Approval Date: 2011

Thesis Language: Turkish

Student: TÜRKAN TURAN

Supervisor: Sezai Türkel

Abstract:

Trehalose is generally over-synthesized under stress inducing conditions and protects S. cerevisiae cells from unfavorable growth conditons. Trehalose is synthesized by TPS enzyme complex. Degredation of trehalose to glucose subunits is catalyzed by trehalase enzyme. Two different trehalase enzymes have been identified in S. cerevisiae. These enzymes are neutral trehalase (Nth1p) and acid trehalase (Ath1p). In this study, the effects of glucose and nitrogen signaling pathway on the transcription of NTH1 gene was investigated. It was shown that the NTH1 transcription is controlled by a similar mechanism in haploid and diploid S. cerevisiae cells. Results of this study also showed that the transcription of NTH1 gene increases several fold in stationary stage and in low glucose medium. It was found that the protein kinase Snf1p regulates NTH1 transcription in response to low glucose signaling. In addition to these results, it was shown that the nitrogen signaling pathway also affects the transcription of NTH1 gene in S. cerevisiae.