The effects of halothane,isoflurane and sevoflurane on the pysiological, some blood biochemical parameters and end tidal CO2 during sponthane and mechanical ventilation in dogs

Thesis Type: Doctorate

Institution Of The Thesis: Uludağ Üniversitesi, Turkey

Approval Date: 2004

Thesis Language: Turkish


Supervisor: AYŞE TOPAL


In this study, it was aimed to compare the effects of three different volatile anaesthetic agents on physiological, some biochemical parameters and end-tidal CO2 (ETCO2 ) parameters during spontaneous and mechanical ventilation in the dogs. Sixty dogs, which in dirferents breeds, sex and weight were used as material in this study. Before the study, health control of each dog was performed and it was determined that all dogs were healthy. Firstly the dogs were divided into three equal group as halothane, isoflurane and sevoflurane groups (n=20). Each group, included 20 dogs, was subdivided into spontaneous and mechanical ventilation groups and procedure of anaesthesia was applied. Physiological parameters, heart rate (HR), respiration rate (RR), temperature (T), mucous membrane colour, capillar filling time (CFT) and blood biochemical parameters Alanine Aminotransferase (ALT), Aspartate Aminotransferase (AST), Gamma-Glutamyl Transferase (GGT), Blood Urea-Nitrogen (BUN), Creatin, Glucose were measured before premedication. Following premedication with Xylazine HC1, anaesthesia was induced with Thiopenthal sodium and beside of above mentioned parameters, mean arterial pressure (MAP), arterial blood gases ([hydrogen ion concentration (pH)], [arterial carbon dioxide pressure (PaCCh)], [arterial oxygen pressure (PaOî)]) and central venous pressure (CVP) were measured. Then inhalation anaesthesia was applied. Oxygen saturation (Sp02 ) was measured by pulse oxymetry and ETCO2 was measured by capnography and values were recorded. Halothane, isoflurane and sevoflurane were used as inhalation anaesthetic agent. Each of the anaesthetic agent was applied both by spontaneous ventilation and also mechanical ventilation. In the spontane ventilation group, animals were allowed to breath IVspontaneously. In the mechanical ventilation group, mechanical ventilation was applied by adjusting tidal volüm 10-20 ml/kg, respiration rate 12-14 per minute and inspiration- expiration rate (I:E) 1 :2. Physiological parameters were measured at 0., 15., 30., 45., 60. and 90. minutes. Blood biochemical parameters were measured prior from premedication and 30., 60. and 90. minutes during anaesthesia. After study, all animals were recoverd from anaesthesia and returned their normal lives. Workfile has been evaluated as statistically. As a result, it was determined that mechanical ventilation is necessary for maintanance of respiration functions during anaesthesia and to provide stable anaesthesia. When we considered all anaesthetic agents, we used, because sevoflurane has positive effects on hepatic, renal, cardiovascular and respiratory systems, it should be chosen with a priority as an anaesthetic agent. And also, it was concluded that routin usage of capnography is very important in the early detection of complications related to anaesthesia and to asses the adequacy of ventilation.