The effect of trout seminal plasma to ram sperms spermatological properties and viability


Thesis Type: Doctorate

Institution Of The Thesis: Bursa Uludağ University, Sağlık Bilimleri Enstitüsü, Turkey

Approval Date: 2006

Thesis Language: Turkish

Student: Meral Gökdağ

Supervisor: MUSTAFA KEMAL SOYLU

Abstract:

Ram sperm containing different ratios of trout seminal plasma and TRIS dilution preserved at 4°C was observed for 72hours with reference to spermatozoon viability, motility, and achrosomatic stability. Pooled sperm was diluted with TALP; TALP and ram seminal plasma removed by centrifuge. Sperm so obtained was divided into 4groups. Group1 sperm were diluted by TRIS diluent at ratio determined by sperm quantity. The remaining three groups of sperm were diluted by trout seminal plasma at respectively 20%, 40%, and 60% and by TRIS as determined by sperm quantity. 2 hours upon dilution, all groups were reduced to +4°C. Samples were obtained respectively after pooling, centrifugation, and diluting, at 4°C and every 12hours thereafter until the 72nd hour inclusive. Samples were examined regarding motility, spermatozoon death, achrosomatic stability. Final data was statistically evaluated by Statistica software. Results are presented in median and standard deviation (±SEM) values, t-test was implemented for median reliability. Confidence Interval Test was implemented for differential analysis and results presented graphically. ANOVA was implemented for identifying varience between control group and 20%, 40%, and 60% seminal plasma added groups. Results were tested by LSD (Least Significant Difference). Motility evaluation after the study had been repeated 10 times, indicated that while the control group yielded better result up to the 24th hour, the 20% trout seminal plasma added group yielded better result after the 24th hour. Spermatozoon death in the 40% group yielded comparatively better result after the 24th hour. In achrosomatic stability yielded better results in the 20% and 40% trout seminal plasma added groups. Furher research is in order using trout seminal plasma with diluents in varying ratios, supported by freezing and fertility experiments in in vivo environment.