Tip 2 diyabet oluşturulmuş sıçanlarda silimarin, oleuropein ve saksagliptinin oksidan–antioksidan sistemler üzerine etkisiThe effect of silymarin, oleuropein and saxagliptin on oxidant-antioxidant systems in type 2 diabetic rats


Thesis Type: Doctorate

Institution Of The Thesis: Bursa Uludağ University, FEN-EDEBİYAT FAKÜLTESİ, BİYOLOJİ, Turkey

Approval Date: 2014

Thesis Language: Turkish

Student: SEDEF ZİYANOK

Supervisor: Sibel Taş

Abstract:

Oxidative stress which occurs as a result of increased blood glucose and lipid levels plays an important role in the progression of the complications of diabetes. Since Olea europaea and Silybum marianum extracts is able to reduce blood glucose and lipid levels, it is thought to decrease oxidative stress. This study was designed to investigate the effects of Olea europaea and Silybum marianum extracts and saxagliptin on hypoglycemic, oxidant and antioxidant systems in streptozotocin -nicotinamide induced type 2 diabetes. Subjects were made type 2 diabetes by injecting nicotinamide (45mg/kg) intraperitoneally 15 min before injection of streptozotocin (65 mg/kg). Saxagliptin (1.5 mg/day) and herbally extracts (1%) was supplemented into drinking water for 5 weeks. Hundred male Wistar rats were randomly divided into ten groups; control (C), control + Olea europaea extract (C + OEE), kontrol + Silybum marianum extract (C + SME), control + Olea europaea a and Silybum marianum extract (C + OEE + SME), diabet (D), diabet + Saxagliptin (D + S), diabet + Olea europaea extract (D + OEE), diabet + Silybum marianum extract (D + SME), diabet + Olea europaea and Silybum marianum extract (D + OEE + SME), diabet + Saksagliptin + Olea europaea and Silybum marianum extract (D + S + OEE + SME). Serum antioxidant enzyme capacity were significantly increased in C + OEE, C + SME and C + OEE +SME groups when compared with control group. Serum trigliseride, total cholesterol levels, blood glucose and tissue malondialdehyde levels were observed to reduced significantly in D + OEE, D + SME and D + OEE +SME and D + S groups while serum antioxidant enzymes (SOD and GSH-Px) activity were significantly increased in D + OEE, D + SME and D + OEE +SME and D + S groups group when compared with diabetic group. In conclusion, because of theirs antihyperglycemic and antihyperlipidemic and antioxidant features, oral antidiabetic agents and herbal extraxcts plays a protective and preventive role against oxidative stress in type 2 diabetes and it can be used to supplement and support the treatment of diabetes.