Effects of glucose precursors on ruminal fermentation and blood parameters in dairy cows


Thesis Type: Doctorate

Institution Of The Thesis: Uludağ Üniversitesi, Turkey

Approval Date: 2019

Thesis Language: Turkish

Student: TOLGA ALTAŞ

Supervisor: HIDIR GENÇOĞLU

Abstract:

In this study; propylene glycol and glycerol, one of the commonly used glucose precursors to meet the increasing energy demand in cows, was aimed to determine which of these two glucose precursors was more effective by observing the changes that dairy cattle had undergone. In this context, the introduction of propylene glycol and glycerol into cows; rumen volatile fatty acids (VFA), ammonia nitrogen, rumen pH values, blood glucose and insulin levels, locomotion score of cows, body condition score and dry matter intake (DMI) were investigated. This research was carried out in the cattle unit in Uludag University, Faculty of Veterinary Medicine, Animal Health and Animal Production Research and Application Center. In this study, 3 dairy cows with black-pied breed who had more than one delivery and a rumen cannula were used. These animals were randomly selected as one animal in each group and divided into three groups as propylene glycol, glycerol and control in 13 day intervals according to 3 x 3 latin square method. The cows were orally administered propylene glycol (300 ml / day), glycerol (450 ml / day) and water (400 ml / day) for a total of 13 days, for 10 days of acclimation and 3 days of sampling. Blood samples at 0 (before drinking) and after 10, 20, 30, 60, 90, 120, 180, 240 and 360 minutes, and rumen fluids at 0 (before drinking) and after taken at 10, 20, 30, 60, 90, 120, 150, 180, 210, 240 and 360 minutes. The amount of dry matter intake by the cows during the study was recorded daily, locomotion score and body condition scores were recorded weekly. SPSS 20 software was used for statistical analysis of the data. In the study, the BMI value of the cows was found to be the highest (22,9 kg / day) in the glycerol-treated group and the lowest (17,3 kg / day) in the propylene glycol-treated group (P <0,02). Blood insulin levels (22,2 ng / dL), glycerol (18,5 ng / dL) and control (16,8 ng / dL) were higher in the propylene glycol-treated group (P <0,05). No difference was found between the propylene glycol and glycerol groups in rumen pH values. However, rumen pH values of propylene glycol and glycerol-treated groups were higher than control group (P <0,05). Similarly, rumen propionic acid values of propylene glycol and glycerol-treated groups were higher than the control group (P <0,05). On the other hand, rumen ammonia nitrogen values were higher in the glycerol-treated group than the control group (P <0,05). From this research; It is concluded that propylene glycol fed to cows increases blood insulin levels, while decreasing DMI significantly, so these effects should be taken into consideration in use.