İnvaziv duktal karsinomlu meme kanserli hastalarda HER2 / HSP90 / SIAH gen ekspresyonlarının retrospektif olarak incelenmesi

Thesis Type: Postgraduate

Institution Of The Thesis: Uludağ Üniversitesi, Turkey

Approval Date: 2019

Thesis Language: English




Among females, breast cancer is the most commonly diagnosed cancer and the leading cause of cancer death. Human epidermal growth factor receptor 2 (HER2) is a member of the epidermal growth factor receptor family that is an important prognostic factor of breast cancer. The introduction of targeted therapies for HER2 has dramatically influenced the outcome of patients with HER2 positive breast cancers. HER2 testing is realized by different methods and it is crucial to standardize testing techniques to evaluate HER2 status accurately. HER2 is a client protein of HSP90, a member of the family of heat shock proteins (HSPs), which are considered molecular chaperones, as they are responsible for the correct folding of denatured or translated proteins. The HSP chaperone activity is tightly regulated by interaction with E3 ubiquitin ligase as seven-in-absentia homolog-2 (SIAHs). In this thesis, we aimed to compare RT- PCR and IHC in the detection of HER2 expression in 94 invasive ductal carcinoma breast cancers, document the level of HER2/ HSP90/SIAH1/SIAH2 expressions in invasive ductal breast cancer, correlate of 4-gene assay expressions with clinicopathological factors, to assess the predictive and prognostic value of the 4-gene assay with RT-PCR in breast cancer patients. We established that HER2 mRNA expressions were coordinated 74,35% with IHC. Our study showed that in HER2-positive (measured with RT-PCR) breast cancers HSP90 and SIAH2 expressions in tumor tissues significantly were up-regulated (p= 0,047; p= 0,024). There was a significant correlation between mRNA expressions of HSP90 and HER2, and also SIAH2 expression was correlated with HSP90 and HER2 expressions