Molecular analysis of the effects of signal transduction pathways on the frameshift in metavirus Ty3

Thesis Type: Postgraduate

Institution Of The Thesis: Bursa Uludağ University, Fen Bilimleri Enstitüsü, Turkey

Approval Date: 2010

Thesis Language: Turkish

Student: Güliz Kaplan

Supervisor: SEZAİ TÜRKEL


Programmed Ribosomal Frameshift is a type of control mechanism which occurs during the translation elongation stage. As a result of Programmed Ribosomal Frameshift, different proteins synthesized with a different ratios from the same mRNA. This control mechanism is commonly used for the synthesis of Retroviral gag and pol polypeptides with a certain ratios and it is also found in different cellular genes of different organisms. Programmed Ribosomal Frameshift can occur at different rates at +1 or -1 directions at different mRNAs. TY3 is present within S. cerevisiae naturally and it replicates via retroviral-like mechanisms within the yeast cells. The effects of glucose and nitrogen signaling pathways on the Programmed Ribosomal Frameshift in TY3 were investigated in this research. It was found that the frameshift rate can vary depending on the growth conditions of the yeast cells. It was shown that the glucose signaling pathway controls the frameshift rate in TY3. It was also shown that the frameshift occurs at very high levels in glycerol lactate grown yeast cells in a protein kinase Snf1p dependent manner. Moreover, it is also found that the frameshift rate is very different in haploid and diploid yeast cells. In addition, it was also shown that the nitrogen signaling pathway has no effect on the frameshift rate in metavirus Ty3.