Determination of genetic diversity of some wheat varieties cultivated in Turkey by RAPD-PCR technique


Thesis Type: Doctorate

Institution Of The Thesis: Uludağ Üniversitesi, Turkey

Approval Date: 2010

Thesis Language: Turkish

Student: ESRA AYDOĞAN ÇİFCİ

Consultant: KÖKSAL YAĞDI

Abstract:

This study was carried out at the Seed Laboratory of the Department of Field Crops, Faculty of Agriculture, University of Uludag in order to determine the genetic diversity of durum ( Triticum durum Desf.) and bread (Triticum aestivum L.) wheat varities developed and cultivated in our country.In this research 14 durum and 16 bread wheats were used. On the basis of RAPD-PCR analysis, out of 45 primers 28 primers for durum wheat and 17 primers for bread wheat were found polymorphic. The polymorphism were determined 66.7 % and 74.9 % for durum and bread wheat, respectively. For durum wheats, polymorphic primers detected 200 bands and 192 (% 64.5) of them polymorphic while total of 142 bands which were amplified with 17 primers out of 110 (% 77.4) were polymorphic for bread wheat. DNA fragment size ranged from 300-4000 bp and 300-2800 bp in respect of durum and bread wheats. Genetic similarity matrix changed into 0.434 to 0.874 (durum wheat) and 0.316 to 0.860 (bread wheat). Using UPGMA (The unweighted pair groupin gmethod of arithmetic averages) to cluster data it was seen that durum wheat varities were grouped into six cluster while bread wheats were into 2 group. The result of this analysis indicated that for durum wheats the highest similarity was between Çakmak-79 and Kızıltan-91 whereas the genetic distance between Ege-88 and Ankara-98 was the lowest and the highest similarity between Gerek-79 and Basribey-95 while Sagittario and Gönen-98 varities were the lowest similiar genotypes for bread wheat.At the result of Principal Component Anlysis (PCA), used for detecting the genetic variation between wheat varieties, 8 groups for durum and 5 groups for bread wheat were determined.The result of the study indicated that the registered varieties in our country, possesed relatively narrow genetic background and concluded that to determine the genetic similarity of genotypes for selecting parents in breeding programmes RAPD technique can be used as a molecular genetic marker.