Isolation of yeasts from Kluyveromyces lactis taxon and characterization of their metabolic and genetic features


Thesis Type: Doctorate

Institution Of The Thesis: Bursa Uludağ University, Fen Bilimleri Enstitüsü, Biyoloji , Turkey

Approval Date: 2009

Thesis Language: Turkish

Student: NÜKHET KAYAKENT

Supervisor: Sezai Türkel

Abstract:

Kluyveromyces lactis is an important industrial yeast species. Different strains of K. lactis are widely used in the production of lactase and also certain recombinant proteins. K. lactis can be found in a variety of habitats. K. lactis species are easily isolated from milk and cheese samples as a pure culture. In this research 6 different K. lactis strains from milk and cheese samples, obtained from local producers in Bursa province, are isolated. In these isolated K. lactis strains their glycogen amounts, lactase and invertase activities are determined. Important differences in lactase and invertase activities of K. lactis strains which are isolated and described as pure culture, are found as compaired with standard K. lactis strain. Some of the structural features of LAC4 and LAC9 genes of these K. lactis strains are also examined. The results show that K. lactis strains isolated from milk and cheese samples obtained from local producers in Bursa province are the new K. lactis strains with their different metabolic properties.