Investigation of the effects of abiotic stress conditions on the transcription of HXT4 gene in S. cerevisiae

Thesis Type: Postgraduate

Institution Of The Thesis: Bursa Uludağ University, Fen Bilimleri Enstitüsü, Biyoloji , Turkey

Approval Date: 2011

Thesis Language: Turkish


Supervisor: Sezai Türkel


Glucose is the preferred carbon and energy resource in S. cerevisiae. Glucose transport into the cell is carried out by the Hxt proteins localized in the cell membrane. Transcription of the HXT4 gene is regulated by glucose signaling and it has a significant function in glucose transport. Once transported into the cell, glucose is metabolized depending on the physiological status of the yeast cell. Under abiotic stress conditions, trehalose and glycerol are synthesized from glycolytic intermediates as stress protectant metabolites in S. cerevisiae. In this research, effects of abiotic stress conditions on the transcription of HXT4 were investigated. It was found that while osmotic stress represses the transcription of HXT4, oxidative stress activates its transcription. On the other hand, it was shown that the temperature stress also activates the transcription of HXT4 gene in certain conditions. In addition to these results, it was found that the stress activated transcription factors Hot1p and Yap1p have no effect on the transcription of HXT4 gene.