Thesis Type: Postgraduate
Institution Of The Thesis: Bursa Uludağ University, Fen Bilimleri Enstitüsü, Moleküler Biyoloji ve Genetik, Turkey
Approval Date: 2016
Thesis Language: Turkish
Student: NACİ ÖZ
Supervisor: Sezai TürkelAbstract:
TOR1 gene of Saccharomyces cerevisiae and its homologs are ser/thr kinases which regulate cellular processes such as cell growth, translation, transcription, lipid and protein synthesis. TOR1 functions in 2 distinct protein complexes, called TORC1 and TORC2. Its major upstream regulators are growth factors, stress, nutrient, cellular energy levels and hypoxia. In this research, conserved regions of TOR1 and its homologs were investigated by using different genome databases. It's found that S. bayanus has overexpression of TOR1 comparing S. cerevisiae according to RNAseq analysis results. To find out its reason, TOR1 promotor regions and transcription factors of these two species were investigated and compared by Deseq2 analysis. It's found that S. bayanus relatively overexpressed certain stress response genes comparing S. cerevisiae. The results of this research revealed that adaptation to different environmental and stress conditions might be linked to TOR signaling pathway.